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cfx384 real time pcr instrument  (Bio-Rad)


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    Bio-Rad cfx384 real time pcr instrument
    Cfx384 Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx384+touch+real+time+system/CFX384+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc13156779-104-28-32
    Average 99 stars, based on 9885 article reviews
    cfx384 real time pcr instrument - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Estimating indoor airborne concentrations of SARS-CoV-2 RNA using quantitative filter forensics
    Article Snippet: This investigation used portable air cleaners (PACs) and quantitative filter forensics (QFF) to assess week-long average airborne concentrations of SARS-CoV-2 RNA in homes occupied with COVID-symptomatic individuals, classrooms, and dining locations throughout Toronto, Canada.. PACs were deployed for one week each, and dust from filters was collected via vacuuming.. SARS-CoV-2 RNA from filter dust was quantified using reverse transcription-polymerase chain reaction (RT-PCR).

    Article Title: A comprehensive analysis of the O-methyltransferase gene family in the chili pepper (Capsicum annuum) identifies COMT36 involved in capsaicinoids biosynthesis.
    Article Snippet: O-methyltransferases (OMTs) play vital roles in secondary metabolites methylation.. While chili peppers (Capsicum annuum) are rich in specialized metabolites, OMT functional characterization remains limited in this species.. In this study, we identified 58 OMT genes through genome-wide analysis, revealing uneven chromosomal distribution with frequent tandem duplications contributing to gene family expansion.

    Expressing:

    Article Title: Influence of preovulatory estradiol treatment on the maintenance of pregnancy in beef cattle receiving in vivo produced embryos.
    Article Snippet: This experiment was designed to determine the role of preovulatory estradiol in pregnancy retention after embryo transfer (ET).. Cows were synchronized with the 7-d CO-Synch + CIDR® protocol.. On d0 (d-2 =CIDR® removal), cows were grouped by estrual status (estrual [Positive Control] and nonestrual), and nonestrual cows were administered Gonadotropin Releasing Hormone (GnRH) and randomly assigned to either no treatment (Negative Control) or Estradiol (0.1 mg estradiol 17-β IM).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Influence of preovulatory estradiol treatment on the maintenance of pregnancy in beef cattle receiving in vivo produced embryos.
    Article Snippet: This experiment was designed to determine the role of preovulatory estradiol in pregnancy retention after embryo transfer (ET).. Cows were synchronized with the 7-d CO-Synch + CIDR® protocol.. On d0 (d-2 =CIDR® removal), cows were grouped by estrual status (estrual [Positive Control] and nonestrual), and nonestrual cows were administered Gonadotropin Releasing Hormone (GnRH) and randomly assigned to either no treatment (Negative Control) or Estradiol (0.1 mg estradiol 17-β IM).

    Real-time Polymerase Chain Reaction:


    Article Title: Epigenetic mechanisms mediate cytochrome P450 1A1 expression and lung endothelial injury caused by MRSA in vitro and in vivo
    Article Snippet: RNA was quantified with a NanoDrop spectrophotometer, and 500 ng‐1 μg of total RNA was used to generate cDNA (High‐capacity cDNA reverse transcription kit, Applied Biosystems, Waltham, MA). .. Quantitative PCR was performed using the CFX384 Touch Real‐Time system (Bio‐Rad, Hercules, CA) utilizing SYBR Green fluorescence (Roche, Mannheim, Germany). ..

    SYBR Green Assay:


    Article Title: Epigenetic mechanisms mediate cytochrome P450 1A1 expression and lung endothelial injury caused by MRSA in vitro and in vivo
    Article Snippet: RNA was quantified with a NanoDrop spectrophotometer, and 500 ng‐1 μg of total RNA was used to generate cDNA (High‐capacity cDNA reverse transcription kit, Applied Biosystems, Waltham, MA). .. Quantitative PCR was performed using the CFX384 Touch Real‐Time system (Bio‐Rad, Hercules, CA) utilizing SYBR Green fluorescence (Roche, Mannheim, Germany). ..

    Variant Assay:

    Article Title: SBSPON gene association with type 2 diabetes mellitus and its impact on leukocyte telomere length
    Article Snippet: .. Variant rs2291219 of the SBSPON gene was genotyped using TaqMan allele discrimination assay on Biorad CFX384 Touch Real-Time System (Bio-Rad Laboratories, Inc.). .. Taqman ® SNP genotyping assays (Primer and Probe labeled with Fam and VIC) supplied by Applied Biosystems (Thermo Fischer Scientific, Pleasanton, CA, USA) and Taqman ® master mix II with UNG supplied by Applied Biosystems (Thermo Fischer Scientific, Foster City, CA, USA).

    Control:

    Article Title: Sex differences in the murine HPA axis after acute and repeated restraint stress
    Article Snippet: Rt-qPcR was performed using the taqMantM Gene expression assay (thermoFisher scientifictM). the taqMan® Gene expression assay primer-probes are listed in table 1 (thermoFisher scientific, #4331182). .. GaPDh and tBP were used as reference genes. all samples were analyzed in duplicates and a no template control was included for each assay. the PcR reaction was performed using the cFX384 touch Real-time system (BioRad, #1855484). .. all data are expressed as mean ± standard error of the mean (seM). all statistical data was obtained using GraphPad Prism (version 9.3.1).

    Fluorescence:

    Article Title: Epigenetic mechanisms mediate cytochrome P450 1A1 expression and lung endothelial injury caused by MRSA in vitro and in vivo
    Article Snippet: RNA was quantified with a NanoDrop spectrophotometer, and 500 ng‐1 μg of total RNA was used to generate cDNA (High‐capacity cDNA reverse transcription kit, Applied Biosystems, Waltham, MA). .. Quantitative PCR was performed using the CFX384 Touch Real‐Time system (Bio‐Rad, Hercules, CA) utilizing SYBR Green fluorescence (Roche, Mannheim, Germany). ..



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